ZFIN Image: Gauvrit et al., 2018, Fig. S1

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Figure Caption

Fig. S1

zebrafish hhex mutants exhibit a delay during PHBC formation but form arterial intersegmental vessels

(a-b) Maximum intensity projections of confocal images of 24 hpf Tg(fli:nEGFP); hhex^+/+ and hhex^-/- embryos after GFP immunostaining. hhex mutants exhibit delayed primordial hindbrain channels (PHBCs) formation (arrowheads). (c-d) Maximum intensity projections of confocal images of 48 hpf TgBAC(etv2:EGFP); hhex^+/+ and hhex^-/- embryos. hhex mutants form arterial ISVs. (e-h) Trunk vasculature of 5 dpf Tg(kdrl:EGFP); Tg(-5.2lyve1b:DsRed); hhex^+/+ and hhex^-/-. hhex mutants form arterial ISVs while vessels from the PCV (vISVs and TD) are mostly absent (arrowheads point to the ventrally positioned TD; asterisks indicate lack of this structure). i) Quantification of vISVs across 10 somites in 5 dpf hhex^+/? (n=8) and hhex^-/- (n=8). Values represent means ± s.e.m. ****P ≤0.0001 by t-test. Scale bars: 100 μm (a-b), 200 μm (c-d), 50 μm (e-h).

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Phenotype details

Acknowledgments

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