IMAGE

Fig. 3

ID

ZDB-IMAGE-180828-20

Genes

TagRFP

Antibodies

Ab7-prox1

Source

Figures for Gauvrit et al., 2018

Image

Figure Caption

Fig. 3

Zebrafish hhex mutants lack lymphatic precursors. a–d Whole-mount views of 36 hpf hhex^+/+ and hhex^−/− embryos immunostained for Prox1 (arrowheads point to Prox1⁺ endothelial cells in the PCV). e Quantification of the number of Prox1⁺ endothelial cells across three somites (from two field of view per embryo) in hhex^+/? (n = 8) and hhex^−/− (n = 6). f–i Whole-mount views of 36 hpf Tg(prox1a:TagRFP); hhex^+/+ and hhex^−/− embryos. hhex mutants exhibit a strong decrease in the number of RFP⁺ cells in the PCV compared to hhex^+/+ (arrowheads point to RFP⁺ endothelial cells in the PCV). j Quantification of the number of RFP⁺ endothelial cells across three somites (from two field of view per embryo) in hhex^+/? (n = 10) and hhex^−/− (n = 4). Values represent means ± s.e.m. ***P ≤ 0.001, and **P ≤ 0.01 by t-test. Scale bars: 50 μm

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.