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Fig. 2

ID: ZDB-IMAGE-180828-14
Source: Figures for Tan et al., 2018

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Fig. 2

Induction of zebrafish primitive and definitive hematopoiesis by MLL-AF9. WISH assays and qRT-PCR analysis of hematopoietic markers in MLL-AF9-injected and control embryos. (a–c) WISH assays of hematopoietic markers (lmo2 and gata1) and a vasculature formation marker (fli) in MLL-AF9-injected and control embryos at 24hpf. (d–h) WISH assays at 48hpf of the definitive hematopoietic stem cell marker c-myb, the erythropoietic marker hbbe1, and the myeloid lineage markers l-plastin, mpy, and lyz. Relative expression of the marker genes for (i) primitive hematopoiesis at 24hpf, (j) hematopoiesis at 48hpf, (k) vasculature formation (flk, fli) at 24hpf, and (k) definitive hematopoiesis initiation (c-myb and runx1) at 48hpf analyzed by qRT-PCR.

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