ZFIN Image: Astone et al., 2018, Fig. S7

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Figure Caption

Fig. S7

CRISPR targeted mutagenesis of the zebrafish yap1 and taz loci.

Single guide RNAs (sgRNAs) were designed to target the first exon of yap1 and the second exon of taz. Sequence alignments between WT and mutant sequences for yap1^bns19 (A) and taz^bns35 (B). The underlined nucleotides are the PAM sequences. (A’ and B’) These indel mutations result in a frameshift and are predicted to encode a truncated protein product as represented in the cartoon. (A’’ and B’’) The genotyping of these alleles can be performed by simple PCR followed by resolution of the WT and mutant amplicons by gel electrophoresis. (C) Representative confocal images of Tg(Hsa.CTGF:nlsmCherry);TgBAC(etv2:EGFP) transgenic embryos in yap and taz mutant background. Higher magnification images correspond to the region demarcated in yellow. (C’) The mCherry fluorescence was measured in the endothelial cells and normalized for the GFP fluorescence used as internal standard. n for each group is indicated. * = p<0.05.

Acknowledgments

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