ZFIN Image: Addison et al., 2018, Fig. 4

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Figure Caption

Fig. 4

Effect of Decreasing the Number of egr2b-Expressing Cells

(A and B) H2B-GFP-labeled wild-type donor cells were transplanted into egr2a plus egr2b morphant hosts (A). Since autoregulation is required to maintain egr2 expression (Figure S2), only the transplanted cells can express egr2 in r3 at this stage. Wild-type donor cells at the position of r3 (denoted r3^∗) in egr2 morphants form a small cluster and maintain egr2b expression at 17 hpf (B, white arrowhead).

(C and D) H2B-GFP-labeled ephA4 morphant donor cells were transplanted into egr2 morphant hosts (C). ephA4 morphant donor cells no longer cluster in r3^∗ but still maintain egr2b expression (D, white arrowheads). Single slices from a confocal z stack are shown. Scale bars: 50 μm.

Acknowledgments

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Reprinted from Developmental Cell, 45, Addison, M., Xu, Q., Cayuso, J., Wilkinson, D.G., Cell Identity Switching Regulated by Retinoic Acid Signaling Maintains Homogeneous Segments in the Hindbrain, 606-620.e3, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell