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Fig. S1 Genotyping of embryos collected from cross of hoxb1a heterozygous parents. Several mutant lines used in this study are not viable as adults. As a result, many embryos used in assays were collected from crosses of heterozygous mutants. To ensure the presence of homozygous mutants in an assayed clutch, embryos were individually genotyped following ISH as outlined in the Methods section. Representative genotyping data for hoxb1a mutant embryos stained with (A) spry1, (B) dusp6, (C) egfl6 and (D) greb1l demonstrate that approximately one quarter of the embryos assayed are homozygous mutant (indicated with asterisks), while 100% of the clutch showed normal staining for the assayed gene. (PDF 967 kb)