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Fig. S5
Subcellular localization analysis of DrDDX41 in zebrafish WBCs and ZF4 cells. (A) Western blot shows that the rabbit anti-DrDDX41 Ab can specifically bind to the corresponding endogenous target proteins in the whole cell lysates of the WBCs sorted from the peritoneal blood, kidney, and spleen. (B) Confocal microscopy image of WBCs stained with rabbit anti-DrDDX41 Ab, showing that the DrDDX41 protein partially distributed in nucleus and cytoplasm in the natural WBCs. WBCs stained with a non-related rabbit IgG was used as negative control. (C) Representative images of transfected ZF4 cells with pEGFP-N1 and pEGFP-DrDDX41. Nuclei were stained with the DNA-intercalating dye DAPI. Scale bars represent 5 μm. Images were captured under a laser scanning confocal microscope (Zeiss LSM-710; original magnification, 630×).