ZFIN Image: Grajevskaja et al., 2018, Fig. S3

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Figure Caption

Fig. S3

Tamoxifen-dependent and heart-specific tg(tnnt2:CreERT2)tpl48 driver can re-mutate tbx5a^tpl58R cardiomyocytes, which are then retained in adult hearts.

Top, diagram of the miniTol2/tnnt2:CreERT2; crygc:mRFP) transgene. Left, diagram of a Cre-inducible mRFP (flox-mRFP) reporter and heart-specific mRFP expression in 4-HT-treated double transgenic fish. Middle panel, Cre-inducible GFP reporter (flox-GFP) and heart-specific GFP expression in double transgenic fish treated with 4-HT. Right, re-mutation of the gene trap in the cardiomyocytes at 3 dpf. Right bottom, heart of an adult fish which was treated with 4-HT between 2–3 dpf displays mosaic mRFP expression in the atrium and the ventricle, indicating mosaicism for tbx5a^tpl58 gene trap re-mutation.

Acknowledgments

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