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Fig. 5-S1
Depletion of Lats1/2 leads to an increase in the expression of hand2.
(A–C) WISH analyses of control embryos (uninjected, left panels, n = 16 to 31) and embryos injected with lats1/2 MOs (right panels, n = 20 to 32) using the antisense probes indicated to the left of the panels at the 10 ss. (A) Square brackets indicate the gap between the hand2-positive regions of the ALPM and the PLPM. Note that the gap is absent in the lats1/2 morphants. (B) Square brackets indicate the presence of tal1 in the rostral end of the PLPM in the control embryos (left), whereas square brackets indicate the absence of tal1 in the lats1/2 morphants (right). (C) Comparable expression of gata4, etv2, nkx2.5, and hoxb5b in control embryos (left panels) and lats1/2 morphants (right panels) at 10 ss. Dorsal view, anterior to the top. WISH images are a set of representative images from four independent experiments.