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Fig. 5
CC and CXC receptors can regulate the migration of primordial cells employing the same downstream signaling pathway.
(A) Epifluorescence image of 10 hpf embryos expressing different ligands (Cxcl12a, Cxcl12b, Ccl25 and Ccl19) in one half of the embryo (mCherry-expressing cells) and PGCs (mGFP) expressing different chemokine receptors (Cxcr4b, Cxcr4a, Ccr9 and Ccr7) along with PTX. Merged images show the position of PGCs with respect to control (cntl), or ligand-expressing cells. (B–E) Graphs showing the quantitation of directed cell migration, by presenting the percentage of PGCs located within the ligand-expressing domains. 60 pg of mGFP-nanos was used to label PGCs in green and 40 pg of m-cherry-globin mRNA was used for labeling the ligand-expressing half of the embryo. 10 pg of PTX-encoding RNA were injected to inhibit the Gi protein. 20 pg of receptor-encoding RNA was used and 30 pg of ligand-encoding RNA was used. 0.2 pmol of cxcl12a morpholino was used. Equimolar amounts of cntl RNA were used. For raw data see Figure 5—source data 1 .