Fig. 4
Liver histological changes of hC7aSP1 zebrafish larvae at different quantities of feeding and lipid contents. The histological changes in the liver of zebrafish larvae fed with HFD (A) for WT and (B) for hC7aSP1. The histological changes in the liver of zebrafish larvae fed with LFD (C) for WT and (D) for hC7aSP1. Zebrafish larvae were analyzed using whole-mount ORO (1, magnification 50×, scale bars: 200 μm), H&E staining (2, magnification 200×, scale bars: 100 μm), H&E staining (3, magnification 400×, scale bars: 20 μm) and frozen section ORO staining (frozen ORO) (4, magnification 400×, scale bars: 10 μm).