Fig. 2
Characterisation of skeletal muscle pathology in neb?/? fish. a Gomori trichome staining of neb?/? skeletal muscle sections reveal the presence of dark regions (arrows) throughout the muscle indicative of nemaline bodies not observed in neb +/+ fish. Nuclei (arrowhead) are evenly organized in neb +/+ , however, appear disorganized in neb?/? fish. b Quantification of normalized fiber area from Gomori trichome stained sections in neb?/? (n?=?23 fibers) compared to neb+/+ fish (n?=?21 fibers). Error bars represent meanąSD, *** p <?0.001. c neb?/? mutants exhibit F-actin (red) and Actinin2 (green) positive aggregates at the myosepta (arrowheads) (and zoomed inset) compared to wildtype siblings at 2 dpf