Fig. 7-S8
Chordacentra always align with the myotome boundaries in wild type larvae, but not in mutants.
(A-R) Live confocal images of entpd5:Kaede in the trunk of wild type, her1−/−;her7−/− and fss (tbx6−/−) larvae, in lateral view, anterior to the left. (A-F) Live images at 4 dpf and 7 dpf show entpd5:kaede positive notochord segments (B and E) are aligned with the myotome boundary (A and D) in wild types. In her1;her7 (G-L) and tbx6 (M-R) mutants at 10 dpf, the myotome boundaries are disrupted and are no longer in strict alignment with the entpd5:kaede segment. Asterisks (*) denote defects in axial segmentation; dashed lines trace myotome boudaries and boudary fragments. Scale bars are 100 µm.