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Fig. 3-S1
osterix is required for the formation of cranial bone structures, but not for the axial skeleton.
(A) Lateral view of a transgenic entpd5:YFP; osterix:mCherry embryo. entpd5:YFP expressing cells are present at positions (arrows; numbers refer to prospective vertebrae 3–5) where mineralization of the vertebral centra are predicted to occur. osterix:mCherry expressing cells are not observed at these positions. (B) Lateral view of sibling and osterix mutant embryos, stained with Alizarin Red. Reduced mineralization seen in the head of mutants, but not around the notochord. (C) Lateral view of sibling and osterix mutant embryos, where all bone elements are labelled with entpd5:kaede. entpd5 expression is reduced in the craniofacial bones of the mutant compared with the sibling. The axial skeletons show no changes in vertebral expression pattern. All images are lateral views, with anterior to the left. Scale bars are 100 µm.