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Fig. 6
myo1D interacts with vangl2 to control cilia orientation in the zebrafish left–right Organizer. a–e myo1D and vangl2 are required for basal body positioning. a–d High magnification views of KV cells (ZO-1) with cilia (acetylated tubulin) and basal bodies (γ-tubulin). e Dot plot representing average antero-posterior basal body position in individual embryos (see Methods). Compared to WT (a, n = 40 embryos/744 basal bodies), basal bodies are displaced anteriorly in MZ myo1D (b, n = 74/1521) or vangl2 (c, n = 36/526) mutants. Basal bodies are similarly affected in MZ myo1D and MZ myo1D ; vangl2+/− (d, n = 37/826). f, g MZ myo1D (n = 93 embryos/3172 cilia) and vangl2 (n = 50/1545) mutations have opposing effects on cilia orientation. Cilia orientation in MZ myo1D ; vangl2+/− (n = 35/1225) is similar to WT (n = 55/2275). h–o two-dimensional dot plots indicating the position of KV cilia pointing to the anterior (red) or posterior (green) in the KV of embryos of different genotypes. Posteriorly pointing cilia invade the posterior KV in MZ myo1D (i), while anterior pointing cilia invaded the anterior KV in vangl2−/− (n). In MZ myo1D ; vangl2+/− (k, o), cilia distribution is again more similar to WT (see Supplementary Fig. 9 for details). All data collected at eight-somites stage, anterior up in a–d, h–o. Horizontal bars in e represent mean values. Error bars in f, g indicate SEM. Scale bar: 5 µm in a–d