Fig. S3
aura mutant embryos exhibit a similar initial number of cortical germ plasm RNPs. Wild-type and aura mutant embryos fixed at 12 mpf show a similar number of RNPs. (A.B) Labeling of RNP with anti-P-myosin. (A’,B’) Reversed grayscale for improved visualization. The RNP zone in the center of the blastodisc is likely the incipient zone of RNP clearing, which our observations suggest moves outward faster in aura mutants than in wildtype. (C) Quantification of the number of spots in image fields using automated particle counting (FIJI) shows no statistically significant difference between wild-type and mutant embryos (8 embryos each). Scale: 100 μM.