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Fig. 7
FMA distal enrichment correlates with SCW directionality. (A-B?) Calcium levels in single Tg[?actin2:GCaMP6s] transgenic embryos during furrow progression, with plotted profiles (A?,B?) corresponding to boxed areas. Wild-type embryos (A?), but not nebel mutants (B?), show increasing distal calcium levels. (C-D?) Microtubules in EMTB::EGFP transgenic embryos during furrow progression (C,D), with plotted profiles (C?,D?). Wild-type embryos (C?), but not nebel mutants (D?), show distal enrichment. Time points T1, T2 and T3 correspond, respectively, to frames within one-tenth of the total sequence of acquired images spanning a furrow formation cycle at the beginning, middle and end of that cycle. See Fig. S4 for additional profiles.