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Fig. 5-S1
isl1 morphants have reduced levels of Isl1 protein and phenocopy isl1 mutants.
(a) At 48 hpf, the heart rate of isl1 morphants (n = 10) is significantly lower than that of uninjected (n = 13) or control morpholino injected (n = 12) embryos, and similar to that of isl1 mutant embryos (n = 14), in agreement with previously published data (de Pater et al., 2009). The heart rate of isl1 mutants that were injected with isl1 morpholino (n = 3) do not show significant difference with that of isl1 mutants or morphants. (b) Antibody staining for Isl1 in 48 hpf Tg(myl7:HRAS-EGFP) hearts (arrowheads) showing decreased levels of Isl1 protein in isl1 morphants and mutants. Altogether, these data suggest that the observed cardiac phenotypes in isl1 morphants are not caused by non-specific effects from the morpholino injections. Student t-tests were performed, *p<0.05; **p<0.005; ***p<0.0005; ns, not significant; error bars indicate ±SD. Scale bars: 100 µm.