ZFIN Image: Hiscock et al., 2018, Fig. S3

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Figure Caption

Fig. S3

A: Left: We compare regions adjacent to a mitotic cell (15 ?m x 15 ?m apical surface, manually contoured) [orange], to nearby control regions of the same dimensions but without an arrested cell [cyan]. Single cell tracking reveals that there is little movement of cells along AP/DV that would take them out of the regions (~2.6?m mean distance moved). Right: segmented image. Asterisks depict an arrested mitotic cell.

B: Illustrative calculation. We define the fraction of neurons as , i.e. for the right . We define the proliferation rate between the two timepoints as: .

(C) There is an increased fraction of neurons adjacent to arrested mitotic cells (red) compared to control regions without an arrested cell (blue) (p < 0.01). Proliferation rates are similar in the two cases (right) (p = 0.9). (n = 7 for both cases)

Acknowledgments

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