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Fig. 3-S2

ID: ZDB-IMAGE-180724-5
Publication: Janjuha et al., 2018 - Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish
All Figures: Figures for Janjuha et al., 2018

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Fig. 3-S2

Fluorescent activated cell sorting of NF-kB:GFP^high and NF-kB:GFP^low beta-cells.

(a) Confocal image of dissociated cells from dissected islets of 3 mpf Tg(ins:nlsRenilla-mKO2) animals that were labeled with TSQ (Zn²⁺ labeling dye). Islets were incubated with TSQ after dissociation and imaged using a confocal microscope. Beta-cells show RFP expression (red) while cells rich in Zn²⁺ are labeled with TSQ (cyan). TSQ strongly labels all beta-cells and weakly labels some unknown endocrine cells. (b) Fluorescent activated cell sorting (FACS) of live TSQ-positive GFP^high and GFP^low cells from Tg(NF-kB:GFP) animals at 3 mpf and 1 ypf. Dead cells were labelled using far-red stain DRAQ7.

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