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Fig. S8
Expression of tph2 in bsx mutants is selectively lost in the pineal gland.
(A) Biochemical steps in the biosynthesis pathway of melatonin from the essential dietary amino acid Ltryptophan, catalyzed by the enzymes Tryptophan hydroxylase (TPH), Aromatic L-amino acid decarboxylase (AADC), Aralkylamine N-acetyltransferase (AANAT) and Acetylserotonin Omethyltransferase (ASMT). (B,C) Dorsal views of embryos at 3 dpf after in situ hybridization against tph2. Anterior is to the left. Scale bar: 100 µm. Expression of tph2 in the pineal gland is completely lost in bsx mutants (arrowhead), while tph2 expression in the midbrain raphe nucleus is normal (asterisk) (n≥6 embryos for each genotype).