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Fig. 5-S2
Proliferating cell nuclear antigen (PCNA) antibody staining shows that NF-kB:GF Phighbeta-cells proliferate less than NF-kB:GFP low beta-cells.
(a) Islets were stained for PCNA to mark the proliferating beta-cells in Tg(NF-kB:GFP) animals at 3 mpf. The confocal image (single plane) shows an overview of a section through the islet. The sections were stained with an insulin antibody (red), a GFP antibody (green) and PCNA (blue). (a’–a’’) The insets show higher magnification images. PCNA can be observed in some of the GFPlow cells (white arrow-heads).
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