ZFIN Image: Kontarakis et al., 2018, Fig. S9

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Figure Caption

Fig. S9

Flt4 but not Vegfr2 inhibition disrupts facial lymphatic development in zebrafish. Tg(lyve1:DsRed2) animals were treated with inhibitors for Vegfr2 (SKLB1002), Flt4 (SAR131675) or DMSO (equivalent to the highest inhibitor concentration) from 48 hpf until 5 dpf (as in Supplementary Figure 8) and facial lymphatic formation was scored at 5 dpf. DMSO controls and Vegfr2 inhibitor treated larvae (2.5 μM or 5 μM) showed no defects. SAR131675 treatment inhibited facial lymphatic formation at both concentrations tested; two severity categories for 2,5 μM are shown, all 5 μM SAR131675 treated larvae completely lacked facial lymphatic outgrowth. Data from 3 independent experiments.

Acknowledgments

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Reprinted from Developmental Biology, 437(2), Kontarakis, Z., Rossi, A., Ramas, S., Dellinger, M.T., Stainier, D.Y.R., Mir-126 is a conserved modulator of lymphatic development, 120-130, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.