Fig. 2
Morphological characteristics of shank3b ?/? larvae and adult zebrafish. a?b Abnormal morphological changes in shank3b ?/? and shank3b +/? larvae at ~?1 dpf, including severe developmental delay, eye melanin reduction (blue arrow), and tail bending (red arrow) (+/+, N?=?60; +/?, N?=?50; ?/?, N?=?50). c?d Normal morphology and body length of shank3b +/+ , shank3b +/? , and shank3b ?/? larvae at 3 dpf (c) and adults (6 mpf, male) (d) (N?=?20 for each genotype). e?f Significantly enlarged brain size (e) but normal brain weight (f) in adult male shank3b ?/? (6 mpf) compared to WT zebrafish (N?=?30 for each genotype). *p?<?0.05