Fig. 2

Characterisation of NBT:GCaMP3 expression in the eye of 7 dpf fish. (A) The NBT:GCaMP3 transgene product, visualised with an anti-GFP antibody (green), sparsely labels HuC/D positive neurons (magenta) that project to the synaptic inner plexiform layer (IPL). Amacrine cells are labelled in the inner nuclear layer (arrows) and retinal ganglion cells are labelled in the ganglion cell layer (arrowheads). (B) Anti-GFP antibodies label only a small number of zn5-positive retinal ganglion cells (arrowhead). GFP-positive amacrine cells in the inner nuclear layer are zn5-negative (arrow). (C) The NBT:GCaMP3 transgene is expressed in a small number of retinal ganglion cell axons in the optic nerve (ON, arrowhead). The blue label is a counterstain. Scale bars in A = 40 µm, B = 50 µm, C = 25 µm.