ZFIN Image: Knickmeyer et al., 2018, Fig. S2

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Figure Caption

Fig. S2

Establishment of a TGF signalling reporter in zebrafish.

(A) Construct for the TGFβ signalling reporter. Multimerised Smad Binding Elements (SBEs) in combination with a minimal promoter (MP) drive membrane localised GFP (GFPcaax). (B) Bright field, fluorescent and merged images of a zebrafish larva expressing the TGFβ signalling reporter construct (21.5 hpf). Note the expression domains in the forebrain (encircled) and the tail. (C) SB431542 reduces TGFβ reporter activity, DMSO treated fish as control. Treatment was administered from 24 hpf to 48 hpf. The inhibitor was renewed during the time of the treatment.

Acknowledgments

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