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Fig. 6
Schematic representation of the experimental protocol for testing vaccine candidates against the reactivation of a latent mycobacterial infection. Fish with a latent M. marinum infection are immunized with a DNA plasmid carrying a GFP-tagged mycobacterial antigen, which allows detection of successful immunizations by fluorescent microscopy. After 5 weeks, the fish are subjected to dexamethasone treatment (10 μg/fish/day) for 4 weeks, followed by determination of bacterial burdens by qPCR.
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