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Fig. S1
Dorsal (A) and lateral (A’) views of a wildtype 48 hpf zebrafish embryo subject to in situ hybridization using the pam sense probe, with the same photographic conditions as in Fig. 1. No signal was observed in the ventricles, otic vesicles or floor plate, confirming the specificity of the signals observed with the antisense probe shown in Fig. 1. (B) Dorsal view of a wildtype 24 hpf embryo subject to in situ hybridization with the pam sense probe; this image was acquired using DIC optics. No signal was observed in any tissue.
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