ZFIN Image: Flanagan-Steet et al., 2018, Fig. 1

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Figure Caption

Fig. 1

BMV109 Labels Cathepsin Proteases in Developing Zebrafish

(A) BMV109 ABP structure.

(B) Schematic of injection strategies. Shown are in-gel analyses of BMV109 reactivity. The bottom gel panels (outlined in red) are higher exposures of low-molecular-weight species; n = 4 experiments. For all gels, equivalence of protein loaded per lane was confirmed using the stain-free method (Experimental Procedures).

(C) BMV109 was injected into the yolk or pericardial space of 1 dpf embryos and incubated overnight (15 hr); n = 4 experiments.

(D) Live confocal analyses of 4 dpf fli1a:EGFP-positive embryos injected pericardially with BMV109 (red, imaging done 15 hr post injection [hpi]). The schematic shows 5 regions (R1–R5) spanning multiple embryonic tissues oriented head to tail. Meckel’s cartilage, lower jaw cartilage; BV, blood vessel. Scale bar, 10 μm. n = 30 embryos from 3 experiments.