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Fig. 3
Representative malformation images of zebrafish for different time points (24, 48, 72 hpf). Letter represents different time point (a 24 hpf; b 48 hpf; c 72 hpf). Number represents different group (1 control group; 2 50 μg/ml of DEN; 3 100 μg/ml of DEN; 4 150 μg/ml of DEN; 5: 200 μg/ml of DEN). There were hatched larvae and unhatched embryos existing simultaneously at 48hpf. TCM turbid embryonic membrane, CE cardiac edema, NM notochord malformation, TH tail hypoplasia, YCD Yolk sac deformity, GR growth retardation
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