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Publication: Earley et al., 2018 - Genetic analysis of zebrafish homologs of human FOXQ1, foxq1a and foxq1b, in innate immune cell development and bacterial host response
All Figures: Figures for Earley et al., 2018

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Fig. 1

Sequence analysis of foxq1a^bcz11 and foxq1b^bcz18 mutations generated by CRISPR-Cas9 targeting.

a Top, DNA and amino acid sequences of the 5’ end of foxq1a coding region. DNA sequencing chromatograms show the expected foxq1a sequence in the wildtype sibling, and a large 95 base pair deletion (red box) in the homozygous bcz11 mutant, which causes a premature stop (black box with an asterisk) at the beginning of the gene. Bottom, DNA and amino acid sequences of the 5’ end of foxq1b coding region. DNA chromatograms show wildtype sequence in foxq1b wildtype sibling, while the homozygous bcz18 mutant carries a 4 bp deletion and 12 bp insertion (red box) causing a nonsense mutation at the beginning of the gene. gRNA, target sites of the guide RNAs used. b Whole mount live imaging of 5 dpf larvae show normal gross morphological development of foxq1a^bcz11 and foxq1b^bcz18 mutants as compared to wildtype siblings (at least 10 animals were analyzed per genotype group).

Figure Data

Phenotype details

Acknowledgments

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