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Fig. 6
Impairing trunk DRG formation attenuates movement-dependent pallial neurogenesis.
(Ai) Dorsal root ganglia (white arrow) and Rohon-Beard neurons (white asterisk) were visualized in Tg(isl2b:mgfp) larvae (scale bar = 40 µm). Treatment with AG1478 from 8 to 30 hpf prevented development of DRG along the trunk in larvae by 3 dpf without affecting RB neuron populations dorsal to the spinal cord (Aii-iii). Earlier treatment with AG1478 did not affect swimming compared to DMSO-treated controls on 8 dpf (B; n = 6). By 9 dpf, restrained larvae in both DMSO (F-G; control n = 6, restraint n = 7) and AG1478 (H-I; control n = 8, restraint n = 6) treatments exhibited fewer pallial PCNA+ cells compared to controls, however, AG1478-treated controls exhibited fewer pallial PCNA+ cells compared to DMSO-treated controls, despite similar swimming behaviour. *p<0.05. Data are represented as mean ± SEM.