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Fig. 1
Responsiveness to Nodal is lost between shield stage and 75% epiboly. (A) Experimental setup. WMISH, whole-mount in situ hybridization. (B,C) rhNodal induces ectopic expression of mesendodermal markers (ntl and sebox) (B) and inhibits the expression of ectodermal markers (sox2 and sox3) (C) during zebrafish embryogenesis. (D) rhNodal ectopically induces ntl within the animal cap region at shield stage but not at 75% epiboly. The number of embryos with the indicated expression pattern among the total examined in two (sebox, sox2, sox3) or four (ntl) biological replicates is shown. All side views are shown with dorsal towards the right. Scale bar: 250 µm.