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Fig. 4
Mutagenetic phenotype of various nuclear localization signal (NLS)-fused Cas9 proteins and N-Cas9-C mRNA for two sites of the tyr gene. Lateral views of wild-type (WT) (A and E) and tyr-target 1- (B and C) or tyr-target 2- injected (F and G) embryos at 48 hpf (hr postfertilization). The phenotypes observed were WT (A and E), mosaic hypopigmentation (B and F), and null-like (C and G). Proportions of embryos with each phenotype (D and H). Scale bars: 0.2 mm.
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