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Fig. 1
Setup for the experimental musculoskeletal loading of adult zebrafish. (a) Swim tunnel used for conducting the exercise experiment. Zebrafish belonging to the exercise group were placed inside the blue chamber during exercise units; white arrows indicate the direction of the water current. Magnified image on the right displays the exercise group during an exercise session. (b) Zebrafish belonging to the exercise group were trained for four weeks at step-wise increasing hours per day (4 h/day in week one, 5 h/day in week two and three, 6 h/day in week four) while control zebrafish did not experience any exercise. Both groups were labeled using fluorescent chromophore calcein submersions before and after the experiment. (c) Schematic depiction of a zebrafish vertebra. Lateral view (left) and transverse cross section (right).