ZFIN Image: Auer et al., 2018, Fig. S1

Image

Figure Caption

Fig. S1

Variability of sheath length, axon diameter of axons analysed, and validation of markers used. Related to Figure 1.

A) Confocal image of an individual mbp:tagRFPt-CAAX myelinating oligodendrocyte in the spinal cord of Tg(mbp:EGFP-CAAX) full transgenic zebrafish at 7dpf. Scale bar: 10μm.

B) Quantification of the length of randomly chosen myelin sheaths in Tg(mbp:EGFP-CAAX) at 7dpf, and the lengths of myelin sheaths formed by three different individual oligodendrocytes.

C) Confocal images of a spinal cord axon expressing cntn1b:mCherry and cntn1b:Nfasca-EYFP in Tg(mbp:memCerulean) full transgenic animals at 4dpf and 6dpf. Nfasca-EYFP only localises to axonal areas that are not covered with myelin and is diffusely expressed along longer non-myelinated axon stretches (arrowheads). Scale bar: 10μm.

D) Quantification of average sheath length along an individual axon relative to the diameter of the axon.

E) Confocal images of Tg(mbp:EGFP-CAAX) full transgenic zebrafish, individual cntn1b:mCherry labelled axons, and Spectral Confocal Reflectance (SCoRe) as label-free method to visualise myelinated axons. Scale bars: 10μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.