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Fig. 4
Fin fold collapse in 72hpf, 7dpf pectoral and median fins of Tg(Inta11:NTR) following metronidazole treatment.
(A-H) Pectoral and (I-L) median fins of Inta11: NTR + MTZ and Inta11: NTR–MTZ control larvae at 72hpf and 7dpf. Inta11: NTR + MTZ larvae display pectoral fin fold collapse at 72hpf (B, F) and 7dpf (D, H), compared to Inta11: NTR—MTZ (A, C, E, G). Note the collapse of the fin fold (red arrows) (B, D). Panels E-H are magnifications of dotted box in panels A-D. Note the appearance of bending actinotrichia fibrils (yellow arrows) in Inta11: NTR + MTZ larvae (F, H) compared to straight actinotrichia (black arrows) in the Inta11: NTR—MTZ larvae (E, G). Inta11: NTR + MTZ larvae display major median fin fold defects at 72hpf (J) compared to Inta11: NTR—MTZ larvae (I). Note the collapse of the fin fold (red arrows) (J). By 7dpf, Inta11: NTR + MTZ larvae continue to show a reduction in median fin fold size compared to Inta11: NTR—MTZ larvae (K), however defects are ameliorated compared to Inta11: NTR + MTZ larvae at 72hpf (J, L). Note the minor folding of distal tip of the median fin (red arrow) (L). ED, Endoskeletal disc; FF, Fin fold. Scale bars: 100μm in A-D, F, H, I-L; 50μm in E, G.