ZFIN Image: Green et al., 2018, Fig. S4

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Figure Caption

Fig. S4

Kaede expression in control groups C-Water and E-Water. Control (nonexposed) larvae and larvae that were exposed initially to 10 ng EE2/L over the period 0-2 dpf were imaged at 3 dpf (A) and the Kaede response then converted fully from green to red fluorescence via UV exposure (B). Both groups of larvae (control and EE2-exposed) were then exposed to embryo water over the period 3-5 dpf (C), 5-7 dpf (D) or 9-11 dpf (E) and imaged on final day of exposure (n=18). Newly generated Kaede expression (green fluorescence) was not observed (there was some auto-fluorescence). All images were acquired by inverted compound microscope (Zeiss Axio Observer) using a 5x objective. A and B images were acquired using GFP, RFP and DIC filters. C, D, and E are presented with the GFP filter only.

Acknowledgments

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