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Fig. S3
Temporal analysis of fluorescence induction after a single 48h exposure exposure to EE2. ERE-Kaede-Casper embryos were exposed to 10 ng/L and 50ng/L EE2 for 48 hours then washed and incubated in embryo water up to 5 dpf. At intervening 24 hour timepoints larvae were imaged to assess new Kaede expression, then immediately exposed to UV light to photoconvert Kaede fluorescence from green to red. All images were acquired by inverted compound microscope (Zeiss Axio Observer) using a 5x objective. Images are presented with the GFP filter only and therefore only 24 hours worth of Kaede expression is shown in each image.