ZFIN Image: van Boxtel et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Ectopic Nodal-Induced Patterning at the Animal Pole Mimics Patterning at the Margin

(A) Schematic of generation of Ndr1-expressing clones. Single blastomeres are injected at the 128-cell stage (2.25 hpf), fixed at germ ring stage (5.7 hpf) and stained using FISH or immunohistochemistry.

(B) Animal views of germ ring-stage embryos containing Ndr1-expressing clones marked with dextran-fluorescein (dex) and stained for indicated markers.

(C) As in (B), but for fgf3 and fgf8a.

(D) Germ ring-stage embryos containing Ndr1-expressing clones, stained by immunohistochemistry for P-Erk and P-Smad2. Single panels are shown and a merge. The merge on the far right shows the Ndr1-expressing cells labeled with dex (white) with staining for P-Smad2 (green).

(E) Schematic representation of gene expression in surrounding Ndr1-expressing clones.

Scale bars, 100 μm. See also Figure S3.

Acknowledgments

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Reprinted from Developmental Cell, 44(2), van Boxtel, A.L., Economou, A.D., Heliot, C., Hill, C.S., Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages, 179-191.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell