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Fig. S1
Impaired caudal fin-fold regeneration in Pten deficient embryos. (A) Embryos from a ptena+/-tenb-/- in-cross were micro-injected at the one-cell stage with synthetic mRNA encoding WT Ptenb (WT), catalytically inactive Ptenb-C124S (CS), or were not injected (-). At 2dpf the caudal fin-fold was amputated and regeneration was assessed after 3 days (i.e. 5dpf, 3dpa), equivalent uncut controls were included (i.e. 5dpf, uncut). All embryos were genotyped. Representative images of micro-injected embryo caudal fin-folds are shown. (B) Uncut caudal fin-fold growth was quantified by measuring the distance from the tip of the notochord to the edge of the caudal fin-fold. The means of uncut caudal fin-fold growth are depicted relative to caudal fin-fold growth of uncut ptena+/+ptenb-/- controls. Means of micro-injected ptena-/-ptenb-/- embryos were compared to non-injected ptena-/-ptenb-/- embryos. Error bars indicate standard error of the mean.