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Fig. S7
Enhanced apoptosis in the caudal fin-folds of live Pten deficient embryos. Embryos from a ptena+/-ptenb-/- in-cross were amputated and allowed to regenerate. Embryos were stained at 2dpa (i.e. 4dpf, 2dpa) or 1dpa (i.e. 3dpf, 1dpa), or equivalent for uncut controls (i.e. 4dpf, uncut; or 3dpf, uncut), with a dye for cells undergoing apoptosis, acridine orange, for 30mins. Representative images of embryo caudal fin-folds at are shown, and the edge of the fin-fold is indicated with a dashed line. Number of embryos showing similar patterns/ total number of embryos analysed is indicated in the bottom right corner. Representative images for embryos stained at 4dpf, 2dpa are shown in Fig. 7C.