Fig. S5
Normal proliferation in the caudal fin-folds of Pten deficient embryos. (A) Uncut embryos from a ptena+/-ptenb-/- in-cross were fixed at 4dpf (4dpf, uncut) in parallel to the embryos depicted in Fig. 5. (B, C) Embryos from a ptena+/-ptenb-/- in-cross were amputated and fixed at 1dpa (i.e. 3dpf, 1dpa), equivalent uncut controls (3dpf, uncut) were included. Embryos were subjected to whole-mount immunohistochemistry using an antibody specific for the cell proliferation marker PCNA (red). The embryos were counterstained with DAPI (blue). Representative images of embryo caudal fin-folds are shown, and in the left panels the edge of the fin-fold is indicated with a dashed line. Number of embryos showing similar patterns/ total number of embryos analysed is indicated in the bottom right corner. The scale bar represents 100?m. (D) PCNA immunofluorescence between the tip of the notochord and edge of the caudal fin-fold at 3dpf was quantified by mean particle count, with thresholding and size restriction to remove background signal. Equivalent uncut controls were also quantified. Means within amputated or uncut groups were compared to ptena+/+ptenb-/- embryos. Significance: * p<0.05; error bars represent standard deviation. Quantification of PCNA immunofluorescence at 4dpf is depicted in Fig. 6B.