Fig. 2

Fig. 2

(A–C) Lateral view; rnf152 was abundantly expressed in the zebrafish embryos at early stages. (D) Image from the animal pole of shield stage demonstrated thin germ ring with embryonic shield (arrowhead). (E–G) Lateral view of the embryos at tail bud, 14 hpf and 18 hpf. (H) Dorsal view of the embryo at 18 hpf found rnf152 transcripts in the eyes, CNS and spinal cord. (I&J) Lateral and (K) anterior view of the embryos at 24 hpf showed rnf152 transcripts were present in the eyes and CNS and some parts of posterior region. (L&M) Lateral view, (N) dorsal view of the embryos at 48 hpf demonstrated that rnf152 transcripts were in the eyes, midbrain and hindbrain boundary (MHB), and rhombomeres. All embryos were collected synchronously from WT zebrafish for WISH analysis at the corresponding stages. Abbreviations: de- Diencephalon, ES- Embryonic shield, Fb-Forebrain, hb-hind brain, PSM- presomitic mesoderm, ret-eye, SC- spinal cord (n=3). Scale bars A-N: 50 μm.