ZFIN Image: Montague et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Vg1 secretion requires Nodal.

(A) Live imaging of Mvg1 embryo injected with 50 pg of vg1-sfGFP mRNA. Scale bar, 17 um. (B) Mvg1 embryos co-injected with 50 pg of vg1-sfGFP mRNA and 50 pg of cyc, sqt or bmp7a mRNA. Scale bar, 17 um. See also Figure 4—figure supplement 1A. (C) Mvg1 embryo co-injected with 50 pg of pH-sensitive fluorescent vg1 (vg1-pHluorin2) and 50 pg of cyc mRNA. Scale bar, 17 um. See also Figure 4—figure supplement 1B. (D) Mvg1 embryo co-injected with 50 pg of vg1-sfGFP mRNA at the 1-cell stage and 10 pg of cyc-RFP mRNA into 1 cell at the 16-cell stage. Scale bar, 100 um. (E) Mvg1 embryos co-injected with 50 pg of vg1-sfGFP mRNA and 50 pg of cyc- or sqt-RFP mRNA. Arrowheads indicate examples of co-localization. Scale bar, 17 um. See also Figure 4—figure supplement 1C.

Acknowledgments

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