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Fig. 2
Generation of miR27b-SP transgenic zebrafish. (A) Cloning of pri-miR-27b and miR27b-SP into LF2.8 or b-Act expression vectors. (B) Red fluorescent images of miR27b-SP expression in the entire body of bC27bSP1 (panel 1, 9 dpf, 40× magnification, scale bars: 200 µm; panel 2, 4 months post fertilization (mpf), 40× magnification, scale bars: 100 mm) and the livers of hC27bSP1 (3, 9 dpf; 4, 4 mpf, 40× magnification, scale bars: 100 mm). (C) Stem-loop RT-qPCR analysis of mature miR-27b of the liver, heart, intestine, brain, eye, adipose and muscle tissues from the four transgenic lines, bC7aSPs (bC27bSP1,2) and hC7aSPs (hC27bSP1,2). n = 5–8 for each groups. * p < 0.01, and ** p < 0.005.
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