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Fig. S1
Neutrophil numbers in the head and brain region of wild type and mecp2- null larvae
(A) Tg(mpx:eGFP)-positive neutrophils were enumerated in the head region of 5 dpf wild type and mecp2-null larvae using stereo fluorescent microscopy (n=12 embryos per condition). (B) Numbers of Tg(mpx:eGFP)-positive neutrophils present in the brain were counted for wild type and mecp2-null larvae at 5 dpf (n=12 embryos per condition). (C) Representative stereo microscopy images of the head region of 5 dpf Tg(mpx:eGFP) wild type and mecp2-null larvae in which the brain tissue has been indicated with a white dotted line. A Mann–Whitney U test was used for statistical analysis (*: p<0.05; ns: not significant). (D) Oligonucleotide morpholino targeting mecp2 expression was injected as previously described by Gao et al. (2015). Numbers of Tg(mpx:eGFP)-positive neutrophils associated with the gastrointestinal tract of 2 dpf control and mecp2 morpholino injected larvae were counted (n≥30 embryos per condition). Student T-test was used for the statistical analyses (***: p<0.001).
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