ZFIN Image: Miles et al., 2017, Fig. 5

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Fig. 5

Over-expression of both grhl3 and grhl2b results in defective patterning and cellular migration. (a–g) Over-expression of grhl3 results in three disparate phenotypes, notably a failure to extend axis (FTEA; a), dorsalisation (b), or mid-axial duplication (c). ISH showing duplication or bifurcation of the neural tube (shh; d), somites (myoD; E), MHB (eng2a, f) and hindbrain rhombomeres (krox20; g) (h–m) Over-expression of grhl2b mRNA (lateral view H; ventral view, i) results in cyclopia, a defect not seen following injection of control (LacZ) mRNA (lateral view J; ventral view, k). This defect is likely due to impaired anterior neural tube extension in embryos over-expressing grhl2b mRNA, as shown by expression of the neural tube marker shh (arrow) by ISH (l–m).

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