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Fig. S5
Characterisation of tight junction formation following knockdown of grhl3 and arhgef19 at 24 hpf. (A-C) The expression of ZO1 (A) and actin (B) in the EVL (C; merged) of WT fish overlying the region of the midbrain-hindbrain boundary (MHB) is unchanged following MO-mediated knockdown of grhl3 (D-F), arhgef19 (G-I) or both grhl3 and arhgef19 together (J-L) at 24hpf. Note increased EVL cell size in both MO:grhl3 (D-F) and MO:grhl3+MO:arhgef19 (J-L) injected embryos.
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