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Fig. S7
Exogenous supply of MCT8 mRNA recovered the expression of genes affected by MCT8 MO to control levels in 25hpf zebrafish. (A) WISH expression analysis of wnt1. Lateral images of brain in control, MCT8 morphant and co-injection of MCT8 mRNA and MCT8 morpholino in zebrafish embryos at 25hpf. (B) WNT1 protein secretion regulator - wls WISH expression, lateral images of brain and lateral images of spinal cord in control, MCT8 morphant and co-injection of MCT8 mRNA and MCT8 morpholino in zebrafish embryos at 25hpf. (C) Fluorescent WIHC expression analysis of DeltaA NOTCH ligand in hindbrain and spinal cord of control, MCT8 morphant and co-injection of MCT8 mRNA and MCT8 morpholino in embryos at 25hpf. (D) WISH expression analysis of of NOTCH pathway target gene her2 in control, MCT8 morphant and co-injection of MCT8 mRNA and MCT8 morpholino in zebrafish embryos at 25hpf. Lateral images of brain and spinal cord of analysed embryos are presented. In A,B,D scale bar represents 100μm. In C scale bar represents 25μm.